Plant production of nanobodies that neutralize the interaction between ACE2 receptor and SARS-CoV-2 spike protein biorxivpreprint USDA_ARS nanobodies SARSCoV2 coronavirus covid COVID19 spikeprotein
By Bhavana KunkalikarSep 7 2022Reviewed by Danielle Ellis, B.Sc. In a recent study posted to the bioRxiv* preprint server, researchers demonstrated the production of nanobodies against severe acute respiratory syndrome coronavirus 2 spike protein.
About the study In the present study, researchers showed the production of nanobodies® in the plant Nicotiana benthamiana against SARS-CoV-2 spike proteins. N. benthamiana plants were grown and utilized at four to five weeks old for the transient expression of nanobodies via plant infiltration with Agrobacterium EHA105. The leaves of N. benthamiana plants were studied under ultraviolet light for green fluorescent protein expression and harvested two days post infiltration . Subsequently, total plant proteins were extracted.
Similar results were also observed when SP-CoV19_his-GFP and SP-his_CoV19-GFP were employed, showed 100% inhibition of ACE2-RBD interaction at 1µg/mL. Furthermore, the mutated sequence of SP-mCov19_his blocked less than 20% at 1µg/mL and 0% at 0.1µg/mL. This indicated that the plant-produced SP-CoV19_his, SP-CoV19_his-GFP, and SP-his_CoV19-GFP inhibited 100% of the interaction between SARS-CoV-2 RBD and ACE2.
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