Ratiometric Fluorescence Approach to Detecting G6PD nanoscience nanomaterials
By Shaheer RehanSep 12 2022Reviewed by Megan Craig, M.Sc. Glucose-6-phosphate dehydrogenase deficiency can lead to multiple health disorders and is a serious concern globally. In a paper published in the Microchemical Journal, a ratiometric fluorescence approach for detecting glucose-6-phosphate dehydrogenase was developed using dual-emission carbon dots and silver nanoparticles.
Genetic mutations in the glucose-6-phosphate dehydrogenase enzyme, which is present in every cell, results in deficiencies of the enzyme. Unfortunately, enzyme assays using these approaches are insufficiently sensitive, and environmental variables can also influence the outcomes. Fluorescent sensors relying on ratiometric measurements may remove errors caused by the environment or equipment, making the results more accurate.
The ratiometric fluorescence techniques perform the sensing process more accurately and precisely by analyzing the ratio of two different intensity emission wavelengths instead of assessing the intensity of just one wavelength. Research Methodology In this study, glucose-6-phosphate dehydrogenase was detected utilizing carbon dots and silver nanoparticles . Carbon dots were employed because they had a straightforward synthesis process.
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