Exploring the effect of an autism-associated mutation on protein movements kumamotou_int SciReports
HeLa and HCT-116 cells were obtained from RIKEN BioResource Research Center . Cells were grown in alpha-modified minimum essential medium supplemented with 10% fetal bovine serum , 100 µg/mL streptomycin, and 100 units/mL penicillin. Cells were cultured under standard conditions at 37 °C in a humidified incubator containing 5% CO. Transfection of HeLa cells with the EGFP-TOP2B expression plasmid was performed using a FuGENE6 reagent according to the manufacturer’s instructions.
In brief, an FV1200-IX83 laser scanning confocal microscope with an oil-immersed 60× objective was used for fluorescence microscopy. For live cell imaging, cells grown on a glass-bottomed dish were placed on a stage top incubator that maintained a humidified atmosphere and 5% CO. Briefly, HeLa cells in a glass bottom dish were transfected with EGFP-TOP2B plasmid DNA. At 48 h after transfection, live cell imaging was performed as described above.
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The 'guardian of the genome' protects against cardiovascular diseaseA team at the Centro Nacional de Investigaciones Cardiovasculares (CNIC), working in collaboration with institutes in the U.S., has demonstrated that acquired mutations in the gene encoding the protein p53 contribute to the development of atherosclerotic cardiovascular disease.
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Presentation of SLE after COVID vaccination in a pediatric patient - BMC RheumatologyBackground The outbreak of severe acute respiratory syndrome coronavirus 2 has had an enormous impact on global health. Vaccination remains one of the most effective interventions for disease prevention. Clinically significant vaccine side effects are uncommon, though autoimmune-mediated disease occurs in a small percentage of vaccine recipients. Systemic lupus erythematosus (SLE) is a multisystem autoimmune disease that is associated with significant morbidity and mortality. Childhood-onset SLE tends to have more severe disease manifestations than adult-onset SLE. In adults, there are a few reported cases of SLE developing soon after coronavirus disease 2019 (COVID-19) mRNA vaccination. Case presentation A 14-year-old previously healthy male developed laboratory and clinical evidence of SLE, including maculopapular malar rash, arthritis, pleuritic chest pain, and class V (membranous) lupus nephritis, 2 days after his third dose of the Pfizer-BioNTech COVID-19 vaccine. The patient’s symptoms improved after initiation of prednisone and mycophenolate mofetil. We also summarize eleven prior case reports describing SLE after COVID-19 vaccine in adults. Conclusion To our knowledge, this is the first reported pediatric patient with new onset SLE following COVID-19 mRNA vaccination. While potential mechanistic links exist between COVID-19 vaccination and SLE development, additional studies are necessary to elucidate the exact nature of this relationship.
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Bakewell bird flu outbreak prompts protection zoneOfficials say the disease has been detected at a premises near Bakewell in Derbyshire.
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In individuals with Williams syndrome, dysregulation of methylation in non-coding regions of neuronal and oligodendrocyte DNA is associated with pathology and cortical development - Molecular PsychiatryMolecular Psychiatry - In individuals with Williams syndrome, dysregulation of methylation in non-coding regions of neuronal and oligodendrocyte DNA is associated with pathology and cortical...
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