Bacterial fragments from leaky gut help drive obesity, study shows TrentUni BMCMedicine
] was used to compute serum levels of LDL cholesterol. Body weight was measured to the nearest 0.5 kg and height to the nearest 1 cm. For those undergoing bariatric surgery, percentage of excess weight loss was calculated according to the following equation: / × 100, and body fat mass was measured using the bioimpedance method .]. Briefly, AT was incubated with collagenase class 1 for 30 min before being filtered through a cotton mesh and centrifuged.
Western blotting was performed as described previously []. In brief, 30ug of protein was loaded onto a denaturing polyacrylamide gel and transferred on to polyvinylidene difluoride membranes . Membranes were blocked in a 5% bovine serum albumin solution, incubated with antibodies against UCP1 and subsequently with secondary antibodies conjugated to horse radish peroxidase. Equal protein loading was confirmed using antibodies against β-Actin .
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New respiratory syncytial virus immunization products in low- and middle-income countries: potential for cost-effective impact on a high burden of disease in young infants - BMC MedicineAs new, efficacious respiratory syncytial virus (RSV) immunization products reach the market, affordable pricing as well as improved estimation of disease burden and the full potential and cost effectiveness of RSV prevention in the hardest hit geographies in low- and middle-income countries are critical to inform country adoption and enable maximum impact against infant disease and mortality globally. The data reported in the special issue underscore the enormous burden, and associated cost, of RSV disease in young infants in several LMICs, including Kenya and South Africa, as well as the potential for RSV maternal vaccines or long-acting monoclonal antibodies, to be cost-effective and possibly even cost-saving.
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Cheilitis in an atopic dermatitis patient associated with co-infection of Staphylococcus pseudintermedius and Staphylococcus aureus - BMC MicrobiologyBackground Atopic dermatitis (AD) is an inflammatory skin condition distinguished by an activated Th2 immune response. The local skin microbial dysbiosis is a contributing factor to the development of AD. The pathogenic coagulase-positive Staphylococcus aureus is the primary species responsible for the progression of AD. Even though Staphylococcus pseudintermedius is an animal-origin pathogen, it is increasingly becoming a source of concern in human diseases. As another coagulase-positive Staphylococci, it is crucial to pay more attention to S. pseudintermedius isolated from the lesion site. Results In our investigation, we presented a case of cheilitis in a patient with atopic dermatitis (AD). We utilized culture and next-generation genomic sequencing (NGS) to identify the bacteria present on the skin swabs taken from the lip sites both prior to and following treatment. Our findings indicated that the predominant bacteria colonizing the lesion site of AD were S. pseudintermedius and S. aureus, both of which were eradicated after treatment. The Multi-locus sequence typing (MLST) of S. pseudintermedius and S. aureus demonstrated coordinated antibiotic susceptibility, with ST2384 and ST22 being the respective types. Although the skin abscess area resulting from S. pseudintermedius infection was significantly smaller than that caused by S. aureus in mice, the expression of cytokines interleukin-4 (IL-4) and interleukin-5 (IL-5) were significantly higher in the S. pseudintermedius-infected mice. Conclusions The S. pseudintermedius strain isolated from the lesion site of the AD patient exhibited a higher expression of IL-4 and IL-5 when colonized on mouse skin, as compared to S. aureus. This observation confirms that S. pseudintermedius can effectively induce the Th2 response in vivo. Our findings suggest that animal-origin S. pseudintermedius may play a role in the development of AD when colonized on the skin, emphasizing the importance of taking preventive measures whe
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Scottish independence campaigners 'having a go at each other', admits SNP MSPKeith Brown said it was 'unfortunate' when different parts of the independence movement attacked each other.
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I could barely keep it together in this free game where you're the president trying to hide that he's an alienFaking It casts you as President Hugh Mann, a normal guy with nothing to hide as he addresses the constituents of his great nation.
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U.N. Paints Stark Picture of Extreme Weather's Human, Economic Toll Over Past 50 YearsWeather disasters over the past half-century have killed over 2 million people, causing $4.3 trillion in economic loss, finds new UN report
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