Telomerase deficiency and dysfunctional telomeres in the lung tumor microenvironment impair tumor progression in NSCLC mouse models and patient-derived xenografts - Cell Death & Differentiation

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Telomerase deficiency and dysfunctional telomeres in the lung tumor microenvironment impair tumor progression in NSCLC mouse models and patient-derived xenografts - Cell Death & Differentiation
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Targeting telomeres could be an effective therapeutic strategy against lungcancer, according to study CNIO_Cancer

). The following experimental groups were generated: DMEM + vehicle ; LLC + vehicle and LLC + 6-thio-dG . In parallel, an in vivo follow-up of survival was performed in both models. For additional details see online supplementary methods.

Animals corresponding to the human xenograft model were euthanized with the same anesthetic combination. Then, primary tumors were resected, fixed in 10% buffered formalin, embedded in paraffin and cut into 3 μm sections for IHQ and IFC. For additional details see online supplementary methods.Hematoxylin and eosin staining was performed for the quantification of lung tumor area and foci, and Sirius Red staining served to evaluate collagen deposition.

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