Spatiotemporal functional assembly of split protein pairs through a light-activated SpyLigation - Nature Chemistry

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Spatiotemporal functional assembly of split protein pairs through a light-activated SpyLigation - Nature Chemistry
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SpyLigation technology uses light to switch on proteins uwmedicine NatureChemistry

We recognize T. Rapp and R. Francis for synthetic advice, J. Shadish for helpful discussion on molecular cloning, R. Gharios for forward-looking conversations, R. Bretherton for providing the SpyTag peptide and transgenic dermal fibroblasts, J. Davis for gifting the HEK-293T cells and S. Edgar for assistance with mass spectrometry.

Part of this work was conducted with instrumentation provided by the Joint Center for Deployment and Research in Earth Abundant Materials. The Thorlabs multiphoton microscope was acquired with and operated under support from the Washington Research Foundation and the University of Washington College of Engineering, Institute for Stem Cell and Regenerative Medicine and Departments of Chemical Engineering, Bioengineering, Chemistry and Biology.Authors and AffiliationsEmily R.

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