The champagne-stained shirt was worn by Sergio Aguero as he clinched his famous last-gasp goal.
The shirt is due to go for auction at Hansons Auctioneers in Etwall, Derbyshire, on 3 November.
David Wilson-Turner, Hansons' head of sports memorabilia, said: "It was arguably the most important goal in the club's history. "Former Manchester City player Mario Balotelli asked Aguero for the shirt as a souvenir but left it behind with his bag. "It was obtained by a former Manchester City kitman who donated it the National Football Shirt Collection which loans sporting memorabilia to museums."Collection owner Neville Evans, from Wales, added: "After Balotelli left the stadium without his bag - and the shirt - the items were transferred to the club's training ground.