Team explores leaf microbiome in perennial bioenergy crops NatureComms
), most of the terpene transcripts discovered through this approach were associated with pigment biosynthesis . The second most consistent class of BGC transcripts on the focal MAGs were non-ribosomal peptide synthase genes, but the majority of these did not have additional annotations beyond the general category. Therefore, the ORF analysis and BGC detection were complementary in results, especially for terpene- and isoprene-related functions.
. These results suggest that the detected and analyzed MAGs can be widely distributed in midwestern agroecosystems and potentially of general importance for perennial crop environments. Fig. 8: Detection of 36 focal MAGs in publicly available metagenomes of bioenergy grasses based on the average number of reads mapped to each MAG.
The sample size was 55 public metagenomes. Data are presented as mean values ± standard error of the mean. Source data are provided as a Source Data file.Here, we report a multi-year seasonal metagenome and metatranscriptome assessment of plant phyllosphere in agricultural field conditions, focusing on the bacterial functions associated with two promising biofuel feedstocks.
There are multiple lines of evidence that the focal MAGs discussed here represent ecologically important lineages in the switchgrass and miscanthus phyllosphere. First, there is ample overlap among these focal MAGs and the core taxa of high abundance and occupancy from our previous 16S rRNA gene amplicon analysis of the switchgrass and miscanthus phyllosphere diversityM9, which is a genus that had high occupancy across fields and over time, as well as several OTUs assigned.
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