A study in Cancer Cell International finds that microRNA-3180 plays a vital role in inhibiting hepatocellular carcinoma cell proliferation and metastasis by suppressing lipid synthesis and transport and is a potential therapeutic target for such patients.
]. However, miRNAs that target both lipid synthesis and transport pathways have not been investigated.
Wild type and mutant predicted 3′-UTR binding sequences of miR-3180 in CD36 and SCD1 were obtained using PCR and cloned into pmir-GLO dual-luciferase miRNA target expression vectors . The primer sequences of the WT and Mut 3’-UTRs are listed in Table S3. WT and Mut 3′-UTR reporters were co-transfected with miR-3180 mimics or a negative control. The transfected cells were collected, and luciferase reporter analyses were performed according to the manufacturer’s instructions .
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