New technique captures unprecedented view of the active brain RockefellerUniv naturemethods
Comparison of root-mean-square error between ground truth and reconstructions obtained using pixel-space Richardson-Lucy reconstruction and MesoLF phase-space reconstructions, for the low- and high scattering scenarios. n = 8 data points represent runs with different simulated raw data. Comparison of the structure similarity index values between ground truth and reconstructions, same underlying data as in , n = 8 different simulation runs.
Comparison of segmentation performance of MesoLF versus CNMF-E in a 2D slice from a MesoLF recording in mouse cortex, depth 100 µm. Green circles: segments that strongly match with the ground truth. Blue circles: segments that only appear in the ground truth. Magenta circles: segments that are not consistent with ground truth. Comparison of precision, sensitivity, and F1-scores for neuron detection performance in CNMF-E and MesoLF segmentation. Same data as in main Fig.
, reproduced here for convenience. Height of bars: Mean. Error bars: s.d. Black circles: n = 5 simulation runs. Top panel: Illustration of 3D volume containing neurons and exhibiting scattering, as used for volumetric segmentation comparisons in remainder of figure. Schematic illustration of segmentation pipelines in CNMF-E and MesoLF . 3D rendering of segmentation results from CNMF-E and MesoLF . Magenta: Ground-truth neurons, green: segments.
Left column: Standard deviation images of simulated LFM movie containing temporally active neurons and a single plane from reconstructed SD image . The brightness values of neurons in the SD image is chosen such that dimmest neuron is 8 times dimmer than the brightest one . Right column: Same as left column with brightness scaled 5× for clarity. Result of morphological segmentation applied to simulated data shown in . Individual segments highlighted in different colors.
South Africa Latest News, South Africa Headlines
Similar News:You can also read news stories similar to this one that we have collected from other news sources.
Developmental trajectory of transmission speed in the human brain - Nature NeuroscienceThis study mapped the developmental trajectory of transmission speed in the human brain by using electrical pulses and intracranial recordings. The authors found that these pulses travel with increasing speeds up to at least the age of 30.
Read more »
Diagnostic classification of childhood cancer using multiscale transcriptomics - Nature MedicineA new multilevel clustering approach applied retrospectively to 13,000 transcriptomes of different tumors reveals a new diagnostic classification of childhood cancers, in some cases allowing a better prediction of disease outcomes.
Read more »
Structural and functional asymmetry of the neonatal cerebral cortex - Nature Human BehaviourWilliams et al. show that structural and functional brain asymmetry is already seen in the newborn brain, but that adult patterns of brain asymmetry are not fully developed.
Read more »
Nature reserve where you can hear rare 'booming' sound across the shallowsWalkers say they hear the baffling sound echoing across the marshes
Read more »
VGLL3 is a mechanosensitive protein that promotes cardiac fibrosis through liquid–liquid phase separation - Nature CommunicationsHeart fibrosis involves a feedback loop where stiffening increases fibrosis-related gene expression in myofibroblasts. Here authors reveal the mechanosensitive nuclear translocation of VGLL3, where it phase separates and promotes collagen production, and show that its knock-out is protective after myocardial infarction.
Read more »
CFTR function, pathology and pharmacology at single-molecule resolution - NatureA structure–function analysis of cystic fibrosis transmembrane conductance regulator shows its two nucleotide-binding domains dimerize before channel opening, and reveals a mechanism through which conformational changes in the channel regulate chloride conductance.
Read more »