Study shows that the translation of protein by microglia supports efficient phagocytosis NatureNeuro
cell. 3D reconstruction using Imaris software of-stack images from an immunostaining for IBA1 , RPS16 ,and DAPI derived from 4-week-old mouse cortex. The first half of the movie shows a standard fluorescence view, while the second half shows a space-filled render on thresholded IBA1, RPS16 and DAPI channels.
3D movie with a 360° view showing ribosomal subunits and protein synthesis within a PeMP that is phagocytosing a neighboring cell. 3D reconstruction displayed with a space-filled rendering algorithm using Imaris software of-stack images from a whole-mount immunostaining for IBA1 , puromycin , RPL10a-eGFP and DAPI derived from 4-week-old mouse cortex. The microglia’s soma is shown at the center, whereas the DAPInucleus in the corner is nearly 100% engulfed by a microglial process.
3D movie with a 360° view showing ribosomal subunits and protein synthesis within a PeMP-NNC. 3D reconstruction displayed with a space-filled rendering algorithm using Imaris software of-stack images from a whole-mount immunostaining for IBA1 , puromycin , RPL10a-eGFP and DAPI derived from 4-week-old mouse cortex. The microglia can be seen with a long PeMP making contact with an IBA1-negative cell.
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