Overview of the importance of temperature control in liquid chromatography experiments, and the emergence of UHPLC as to be described at Pittcon 2019.
Sponsored Content by PolyScienceAug 31 2023 High performance liquid chromatography and, more recently, ultra-high-performance liquid chromatography are mainstay separation techniques. This is especially true for pharmaceutical, medical, food and industrial research where good separation is linked to the efficiency of the mass spectrometry used in detection and identification of components of interest.
Of course, this is not the same for all compounds but variation of the column temperature can change peak spacing or elution order and cause peak broadening or peak distortion. Poor mobile phase and column temperature control can lead to artefact ghost peaks, increased baseline noise and bubbles of vapour going through the detector.
UHPLC at Pittcon 2019 An Pittcon 2019, UHPLC and temperature control was a topic of major interest. There has also been increased interest in using temperature gradients in LC separations, but these need to be very well controlled using thermal management and thermal sensors. The Waters symposium at Pittcon 2020 examined the origins of UHPLC and the advances in LC instrumentation over the past twenty years with input from experts such as Jorgenson, Bouvier, Barber and Carr.
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