Nature research paper: In vitro production of infectious Plasmodium falciparum sporozoites
transformed Pf zygotes and S2 feeder cells were added to Matrigel matrix coated wells of 8-well chamber slides and 24 h or 36 later an anti-Pfs25 mAb was added to the wells and the parasites were assessed by immunofluorescence assay.. At 24 h, Pfs25 staining of an ookinete and the trail it had shed moving across the well are shown.. At 36 h, Pfs25 staining of an early oocyst and the trail it had shed indicating the likely path the ookinete took before transforming to an oocyst are shown.
signals not localized to oocysts indicate feeder cell nuclei.produced 3-day oocysts expressing Pf25 and 8-day oocysts expressing PfCSP were detected in numerous experiments in 8-well chamber slides with Matrigel.mosquitoes were fed a blood meal containing stage V Pf gametocytes. At 3 days and 8 days the midguts were dissected and stained with anti-Pfs25 mAb and anti-PfCSP mAb and assessed by immunofluorescence using confocal microscopy..
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