Study links metabolism changes in certain brain cells to Huntington's disease NatureComms
under both positive and negative ionization modes in conjunction with referencing of appropriate internal standards: PA 14:0/14:0, PC 14:0/14:0, PE 14:0/14:0, PG 15:0/15:0, PI 17:0/20:4, PS 14:0/14:0, BMP 14:0/14:0, APG 14:0/14:0, LPC 17:0, LPE 14:0, LPI 13:0, Cer d18:1/17:0, SM d18:1/12:0, dhSM d18:0/12:0, GalCer d18:1/12:0, GluCer d18:1/12:0, LacCer d18:1/12:0, D7-cholesterol, CE 17:0, MG 17:0, 4ME 16:0 diether DG, D5-TG 16:0/18:0/16:0 .
Western blot analysis of OPC cultures was performed as outlined above with the following modifications. The following antibodies were used: rabbit anti-PRC-epsilon , mouse anti-OLIG2 , mouse anti-CNPase , rabbit anti-MOG and mouse anti-αTUBULIN . Detection of target proteins was done by measuring chemiluminescence signal using ECL™ Prime Western Blotting Detection Reagent on a ChemiDoc Imaging System . Image J was used to quantify the protein bands and αTUBULIN was used as loading control.
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