Science, Space and Technology News 2024
A new study introduces a technique using artificial spike-ins to improve the accuracy of gene activity analysis in plants, addressing the challenge of global transcription changes. This method provides clearer insights into how plants respond to environmental stimuli, such as temperature, and is hoped to be widely adopted in plant science research. Credit: SciTechDaily.comof techniques that help us understand how external variables – such as temperature – affect gene activity in plants.
“We use RNA-seq analysis to assess how plants respond to various stimuli, or changes in their environment,” Doherty says. “It’s used widely because it’s a relatively easy and inexpensive way to monitor plant responses.” In other words, researchers want to see how specific variables – such as increased temperature – affect transcription in specific genes. But there are some variables – like time of day – that can increase or decrease transcription inthe genes. This can throw off researchers’ ability to draw conclusions about the specific variables they want to study.that they have developed a method to account for it, called an artificial spike-in,” Doherty says.
“The artificial spike-in gave us more accurate information and greater insight into how plants are behaving at night – since we found that global transcription was higher at night. Before we adopted the use of artificial spike-ins, we were missing a lot of what was happening at night.
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