Quick-closing valve allows fish to rapidly regulate the water in their cells
Yeast clone selection was performed on YPD plates containing 2,000 μg/ml Zeocin , and small-scale protein production was tested for selected strains. Membrane fractions were analyzed by SDS–PAGE and immunoblot. Strains with the highest production of His-tagged proteins were picked for large-scale production in 3-liter fermentors. For untagged-cpAQP1aa-243, the strain corresponding to the largest colony on the high-Zeocin plate was used for fermentation directly.
Membrane solubilization of cpAQP1aa-FL was carried out by mixing the membrane with solubilization buffer in a 1:1 volume ratio supplemented with Complete EDTA-free protease inhibitor . The mixture was gently rocked for 1 h in a cold room. Unsolubilized material was removed by ultracentrifugation , and the supernatant was transferred to a falcon tube containing pre-equilibrated Ni-NTA agarose beads and rocked for 3 h or overnight.
For the untagged protein, the solubilization buffer contained 20 mM Tris–HCl , 10% glycerol, 4% NG, and 2 mM β-MeOH. After solubilization and centrifugation, the solubilized material was diluted in a 1:3 ratio with dropwise addition of pre-chilled dilution buffer . The diluted sample was filtered using a 0.2-μm bottle top filter and applied to a Resource S cation exchange column pre-equilibrated with buffer A at a flow rate of 0.5 ml/min.
South Africa Latest News, South Africa Headlines
Similar News:You can also read news stories similar to this one that we have collected from other news sources.
USDCAD clings to gains above 1.3400, over one-week high amid bearish oil prices/stronger USDUSDCAD clings to gains above 1.3400, over one-week high amid bearish oil prices/stronger USD – by hareshmenghani USDCAD China Coronavirus Fed Currencies
Read more »
USDJPY climbs to one-week high, eyes 141.00 mark amid modest USD strengthThe USDJPY pair gains some positive traction for the fourth straight day on Monday and climbs to a one-week high during the early European session. Th
Read more »
China Records First Covid-19 Deaths Since May as Cases Edge Toward Record HighChina acknowledged its first deaths related to Covid-19 in about six months as surging cases collide with a government push to ease unpopular and costly pandemic controls
Read more »
Deadly Indonesia Earthquake Shakes High Rises In Jakarta | Weather.comA deadly earthquake shook parts of Indonesia severely enough to be felt in the country's capital of Jakarta. Here's the latest. - Articles from The Weather Channel | weather.com
Read more »
Red Velvet's Yeri cast in upcoming OTT drama 'Cheongdam International High School' | allkpopYeri is gearing up to star in a new drama!On November 21 KST, SM Entertainment confirmed that the Red Velvet member will be appearing in the upcomin…
Read more »
Scientists develop high-density, all-3D printed Li-metal batteryResearchers at the Chinese Academy of Sciences have developed all-3D printed lithium metal batteries with robust cycle stability and ultrahigh areal energy density.
Read more »