An antibody specific to neurons provides a visualization of different levels of the peripheral nervous system in a whole mouse.
posted eye-catching videos of “flying” through the 3D anatomy of a mouse with different tissues labeledwildDISCO can use various antibodies to map different cell types in dead mice, showing new connections between tissues and much more.The method is a variation of work his team started more than a decade ago, when various scientists were developing novel ways, such as
. Ertürk and colleagues developed a similar method that made entire mouse bodies transparent. Seeking a way to pick out different cell types in the see-through bodies, they turned to nanobodies, synthetic antibodies that are much smaller than standard ones and can slip more easily into the tissue. But only a handful of nanobodies have been designed for specific cell types, limiting efforts to map the mice bodies.
“This is exciting, and the immunolabeling data are quite impressive,” says Alain Chédotal, a neuroscientist at the Vision Institute in Paris who was not involved in the study. So are the fly-through visualizations of the data, he says. “This study also nicely shows that virtual reality is a great way to navigate through large 3D image data sets.”
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