Nature research paper: Fast and sensitive GCaMP calcium indicators for imaging neural populations
. b. Raw fluorescence intensity from the five sensors tested. Inset below: XCaMP-Gf shown with y-axis ~30x smaller. c. Mean intensity over the 0.5 Hz stimulation period shown in b. Kruskal-Wallis test finds P = 5.7e-5 and pairwise Dunn’s multiple comparison test to jGCaMP7f as follows: jGCaMP8f = 6.5e-5, jGCaMP8m = 1.4e-2, jGCaMP8s = 0.37, and XCaMP-Gf = 2.8e-5; totalfor each variant: j7f, 14 flies; jGCaMP8f, 14; jGCaMP8m, 11; jGCaMP8s, 11; XCaMP-Gf, 4.
a. Western blot analysis comparing protein expression between GCaMP variants. Ratio is the band intensity levels from a variant divided by the band intensity from the actin loading control. Multi-comparison Kruskal-Wallis finds=0.038 and pairwise Dunn’s multiple comparison test to jGCaMP7f as follows: jGCaMP8f = 0.011, jGCaMP8m = 0.019, jGCaMP8s = 0.024, and XCaMP = 0.038. Numbers tested are as follow: jGCaMP8f = 3, jGCaMP8m = 3, jGCaMP8s = 3, jGCaMP7f = 5, and XCaMP = 3.
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