Protein partnership effectively fends off pancreaticcancer progression
). The following primers were used: PRDM16-For 5′-CATCTCCCCAGCATTGTCAGT-3′; PRDM16-Rev 5′-GGAGCGCCGAACACGGAATG-3′; JUNB-For 5′-GGCAAAGCCCAGGGTCAATA-3′; JUNB-Rev 5′-AAAGCTAGTAAGCGGCCTGG-3′; GAPDH-For 5′-CGGGATTGTCTGCCCTAATTAT-3′; GAPDH-Rev 5′-GCACGGAAGGTCACGATGT-3′.PANC-1 cells were plated in 6-well plates and transfected with the CAGA9-Lux or Prdm16-Lux reporter in the presence of pcDNA3.1-Prdm16, pCMV5-HA-Smad4, or empty vector using X-tremeGENE9 .
To quantify Prdm16 expression in mouse tissues, slides chosen in a random manner from all mice under study were scanned using the PlanApo 10 × 0.45/4.00 mm objective on the Keyence BZ-X810 automated microscope and characterized using the BZ-X800 Analyzer software from Keyence. Random images of PanIN and PDAC lesions were taken and quantified only using the area directly around the lesions.
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