Transplantation of a plant-derived photosynthetic system, camouflaged with a mature cell membrane, can correct energy imbalance and restore cellular metabolism in chondrocytes. NBThighlight
. In brief, consecutive fractions were collected for liquid chromatography–tandem mass spectrometry analysis. To determine the biological and functional properties of all identified proteins, the identified protein sequences were analysed on the basis of GO terms.RAW 264.7 mouse macrophages, a HUVEC line and a mouse fibroblast cell line were obtained from the China Center for Type Culture Collection.
The fluorescence intensity in each well was quantitatively determined by flow cytometry . FlowJo was used for flow cytometry analysis.Equal amounts of chondrocytes , NPCs , SCs , macrophages and fibroblasts were cultured on Petri dishes and incubated overnight. CM-NTUs at a concentration of 2 × 10NTUs per cell were added and incubated with these cells for 6 h. Then, flow cytometry was performed.
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